02348nas a2200361   4500000000100000008004100001260001600042653001700058653001800075653002000093653002400113653001400137653002600151653001100177653001800188653001400206653002500220653001800245100001300263700001400276700001300290700001300303700001600316700001300332700001700345700001500362700001800377245016400395300001200559490000800571520139300579022001401972       1993                            d  c1993 Jun 0110aAntigens, CD10aCell Adhesion10aCells, Cultured10aChemotactic Factors10aCytokines10aEndothelium, Vascular10aHumans10aInterleukin-110aMonocytes10aRecombinant Proteins10aT-Lymphocytes1 aTaub D D1 aLloyd A R1 aConlon K1 aWang J M1 aOrtaldo J R1 aHarada A1 aMatsushima K1 aKelvin D J1 aOppenheim J J00aRecombinant human interferon-inducible protein 10 is a chemoattractant for human monocytes and T lymphocytes and promotes T cell adhesion to endothelial cells.  a1809-140 v1773 a<p>The human cytokine interferon-inducible protein 10 (IP-10) is a small glycoprotein secreted by activated T cells, monocytes, endothelial cells, and keratinocytes, and is structurally related to a family of chemotactic cytokines called chemokines. Although this protein is present in sites of delayed-type hypersensitivity reactions and lepromatous leprosy lesions, the biological activity of IP-10 remains unknown. We report here that recombinant human IP-10 stimulated significant in vitro chemotaxis of human peripheral blood monocytes but not neutrophils. Recombinant human IP-10 also stimulated chemotaxis of stimulated, but not unstimulated, human peripheral blood T lymphocytes. Phenotypic analysis of the stimulated T cell population responsive to IP-10 demonstrated that stimulated CD4+ and CD29+ T cells migrated in response to IP-10. This resembles the biological activity of the previously described T cell chemoattractant RANTES. Using an endothelial cell adhesion assay, we demonstrated that stimulated T cells pretreated with optimal doses of IP-10 exhibited a greatly enhanced ability to bind to an interleukin 1-treated endothelial cell monolayer. These results demonstrate that the IP-10 gene encodes for an inflammatory mediator that specifically stimulates the directional migration of T cells and monocytes as well as potentiates T cell adhesion to endothelium.</p>  a0022-1007