01959nas a2200349   4500000000100000008004100001260001700042653001500059653001000074653001200084653002400096653001500120653001600135653002000151653001300171653001100184653001300195653001200208653002600220653001600246653001800262653002400280653002500304653003100329100001200360245011300372856004300485300001100528490000700539520104900546022001401595       1978                            d  c1978 Jul-Dec10aAdolescent10aAdult10aAnimals10aAntigens, Bacterial10aArmadillos10aBCG Vaccine10aCells, Cultured10aEpitopes10aHumans10aLepromin10aleprosy10aLymphocyte Activation10aLymphocytes10aMycobacterium10aMycobacterium bovis10aMycobacterium leprae10aMycobacterium tuberculosis1 aBjune G00aComparison of various preparations of Mycobacterium leprae and other mycobacteria by lymphocyte stimulation.  uhttp://ila.ilsl.br/pdfs/v46n3-4a11.pdf  a386-930 v463 a<p>Peripheral blood lymphocytes were stimulated in vitro with different mycobacterial antigen preparations and responses were measured as incorporation of tritiated thymidine. Blood donors were 9 patients with lepromatous leprosy, 16 tuberculoid leprosy patients, and 6 healthy individuals with different degrees of exposure to leprosy. The results revealed a good correlation between the responses to M. leprae from human sources and bacilli from armadillos inoculated with human leprosy bacilli, although the latter were less potent stimulators. Responses to BCG and PPD did not correlate with lymphocyte responses to human M. leprae. Acid-fast bacilli grown on LA-3 medium inoculated with human leprosy bacilli stimulated responses which did not correlate with responses to human M. leprae when positive responses were compared, but showed a good correlation when inhibitory effects of the bacilli on thymidine incorporation were studied. The significance of the lymphocyte stimulation test for identification of microbes is discussed.</p>
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