02098nas a2200409   4500000000100000008004100001260001300042653001500055653001000070653000900080653002600089653002900115653002400144653002700168653003800195653001600233653001100249653002100260653002100281653001200302653001600314653002500330653003100355653002500386100001800411700001300429700001100442700001200453700001400465245015200479856004100631300001000672490000700682050001800689520096700707022001401674       1993                            d  c1993 Mar10aAdolescent10aAdult10aAged10aAntibodies, Bacterial10aAntigen-Antibody Complex10aAntigens, Bacterial10aChemical Precipitation10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aMiddle Aged10aMycobacterium leprae10aMycobacterium tuberculosis10aPolyethylene Glycols1 aYamashita J T1 aCruaud P1 aPapa F1 aRotta O1 aDavid H L00aCirculating immune complexes in leprosy sera: demonstration of antibodies against mycobacterial glycolipidic antigens in isolated immune complexes.  uhttp://ila.ilsl.br/pdfs/v61n1a08.pdf  a44-500 v61  aYAMASHITA19933 a<p>Circulating immune complexes (CIC) were assayed in sera of leprosy patients. Using an immunoassay for two mycobacterial antigens--phenolic glycolipid-I (PGL-I) and glycolipid IV (SL-IV)--sera from 65 patients with leprosy (38 lepromatous, 18 borderline, and 9 tuberculoid) were studied. The CIC were isolated by polyethylene glycol (PEG) precipitation, washed, treated with an acid buffer, neutralized, and tested using an enzyme-linked immunosorbent assay (ELISA). We demonstrated that CIC could contain IgG and IgM antibodies reacting against PGL-I and SL-IV. The high levels of antibodies in the precipitable CIC showed concordance with high levels in the original sera, although some patients presented high levels of precipitable CIC in the absence of high titers of antibodies in their sera. It was concluded that some of the CIC observed in patients with leprosy were composed of IgG and IgM immunoglobulins against specific mycobacterial antigens.</p>  a0148-916X