02410nas a2200385   4500000000100000008004100001260001300042653002400055653002300079653003000102653001100132653002100143653002300164653001200187653002400199653002500223653002500248653002000273653002600293653002500319653001800344653001500362100001500377700001900392700001300411700001500424700001200439700001500451245016500466856004100631300000900672490000700681520132200688022001402010       1993                            d  c1993 Mar10aAntigens, Bacterial10aBacterial Vaccines10aDrug Therapy, Combination10aHumans10aInterferon-gamma10aLeprostatic Agents10aleprosy10aLeprosy, Borderline10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aLeukocyte Count10aLymphocyte Activation10aMycobacterium leprae10aT-Lymphocytes10aTuberculin1 aShinde S R1 aChiplunkar S V1 aButlin R1 aSamson P D1 aDeo M G1 aGangal S G00aLymphocyte proliferation, IFN-gamma production and limiting dilution analysis of T-cell responses to ICRC and Mycobacterium leprae antigens in leprosy patients.  uhttp://ila.ilsl.br/pdfs/v61n1a09.pdf  a51-80 v613 a<p>Lymphocyte proliferative responses and interferon-gamma (IFN-gamma) production after stimulation with antigens of ICRC, Mycobacterium leprae, and purified protein derivative (PPD) were assessed in leprosy patients and healthy donors. The patients studied were newly diagnosed as having lepromatous leprosy (LL), multidrug therapy (MDT) responders (MDT-R LL), MDT nonresponders (MDT-NR LL), borderline lepromatous (BL), and borderline tuberculoid/tuberculoid (BT/TT) leprosy. The tuberculoid leprosy patients showed increased lymphocyte proliferation and IFN-gamma production in response to stimulation with ICRC, M. leprae, and PPD antigens compared to other groups of LL patients and healthy donors. Although lymphocytes from LL patients showed low responses to ICRC and M. leprae antigens, their responses to PPD were not grossly affected. MDT-R LL patients showed higher lymphocyte proliferative responses and IFN-gamma production after stimulation with ICRC and PPD but not with M. leprae antigens. Tuberculoid leprosy patients showed higher T-cell frequencies to ICRC and M. leprae antigens compared to MDT-R LL and MDT-NR LL patients. The increased lymphocyte proliferative responses to ICRC observed in the MDT-R LL patients was reflected in the increased T-cell frequency to ICRC compared to M. leprae.</p>  a0148-916X