01307nas a2200253   4500000000100000008004100001260001700042653001200059653002000071653001800091653001100109653001200120653000900132653002500141653000900166100001500175700001400190245006100204300001100265490000700276050003200283520072400315022001401039       1993                            d  c1993 Apr-Jun10aAnimals10aCells, Cultured10aCulture Media10aHumans10aleprosy10aMice10aMycobacterium leprae10aSkin1 aBhatia V N1 aThawani G00aObservations on attempted leprosy cultures in two media.  a163-710 v65  aInfolep Library - available3 a<p>Suspensions of skin tissue material collected from lepromatous leprosy patients and material from mouse foot-pad harvests were inoculated into two media, viz., a biphasic medium and a minimal basal medium. The cultures were incubated at 37 degrees C and 15 degrees C. Small oval (or round) cells appeared in these cultures around the tenth day along with a few cystic structures; and they increased in number later, reaching the maximum around six-seven weeks. The above cells appeared acid-fast in some cultures and some of them appeared to split into pairs of acid-fast bacilli. The cells were most often seen in the biphasic medium at 37 degrees C. The identity of these structures is not known at this stage.</p>  a0254-9395