02230nas a2200421   4500000000100000008004100001260001300042653001200055653001700067653001700084653001400101653001100115653001900126653002800145653002100173653001200194653002600206653000900232653002400241653001500265653002500280653003700305653001100342653001800353100001400371700001500385700001100400700001500411700001500426700001700441700002000458245011300478856004100591300001200632490000700644520114300651022001401794       1993                            d  c1993 Sep10aAnimals10aCD4 Antigens10aCD8 Antigens10aCell Line10aFemale10aFlow Cytometry10aImmunotherapy, Adoptive10aInterferon-gamma10aleprosy10aLymphocyte Activation10aMice10aMice, Inbred BALB C10aMice, SCID10aMycobacterium leprae10aSpecific Pathogen-Free Organisms10aSpleen10aT-Lymphocytes1 aAzouaou N1 aGelber R H1 aAbel K1 aSasaki D T1 aMurray L P1 aLocksley R M1 aMohagheghpour N00aReconstitution of Mycobacterium leprae immunity in severe combined immunodeficient mice using a T-cell line.  uhttp://ila.ilsl.br/pdfs/v61n3a04.pdf  a398-4050 v613 a<p>To test whether Mycobacterium leprae-immune T cells can confer protection against infection with leprosy bacilli, severe combined immunodeficient (SCID) mice were reconstituted with a BALB/c-derived, M. leprae-responsive, T-cell line. Flow cytometric analysis of spleen and peripheral blood cells confirmed reconstitution with T cells. In vitro lymphokine production and the proliferation of spleen cells from the reconstituted animals established that the donor cells had maintained their functional activity for the duration of the study (275 days). The transfer of immune T cells 24 hr before foot pad infection with leprosy bacilli resulted in a profound reduction in M. leprae multiplication, as compared to the nonreconstituted SCID mice. The yield of acid-fast bacilli in the foot pads of SCID mice reconstituted with the M. leprae-immune T cells also was significantly lower than that found in naive BALB/c mice, and at levels previously found only in BALB/c mice that had been immunized effectively. These experiments demonstrate that M. leprae-immune T cells home effectively and control M. leprae infection in SCID mice.</p>  a0148-916X