01707nas a2200289   4500000000100000008004100001260001300042653001200055653002400067653001800091653002300109653001600132653001100148653003000159653001200189653002100201653002500222653003100247653001800278100001300296700001600309245016000325300001100485490000800496520089900504022001401403       1993                            d  c1993 Nov10aAnimals10aAntigens, Bacterial10aB-Lymphocytes10aBacterial Vaccines10aGuinea Pigs10aHumans10aHypersensitivity, Delayed10aleprosy10aMolecular Weight10aMycobacterium leprae10aMycobacterium tuberculosis10aT-Lymphocytes1 aYadava A1 aMukherjee R00aAn immunodominant 30-kDa antigen of a candidate anti-leprosy vaccine, Mycobacterium w, shares T and B cell determinants with M. leprae and M. tuberculosis.  a243-530 v1823 a<p>Earlier we reported that vaccination of leprosy patients with Mycobacterium w induces an immune response directed predominantly against low molecular weight antigens. One of these antigens, with a molecular mass of 30-kDa, was recognized by a majority of the vaccinated subjects as well as the tuberculoid leprosy patients and healthy contacts. In the present communication we report further characterization of this antigen. Immunofluorescence and Western blot studies with antibodies raised against this antigen demonstrate that it is associated with the cell surface and has homologues present in M. leprae and M. tuberculosis. Delayed-type hypersensitivity studies carried out in guinea pigs immunized with the 30-kDa antigen show that in addition to sharing B cell determinants, this immunodominant antigen of M. w also shares T cell determinants with M. leprae and M. tuberculosis.</p>  a0300-8584