02879nas a2200385   4500000000100000008004100001260001300042653000900055653002200064653002600086653001700112653002800129653003800157653001100195653001100206653002100217653002100238653001200259653000900271653001600280653002000296100001100316700001200327700000900339700001500348700001200363700001100375700001600386700001400402245014400416300001100560490000700571520190100578022001402479       1999                            d  c1999 Nov10aAged10aAged, 80 and over10aAntibodies, Bacterial10aCord Factors10aDiagnosis, Differential10aEnzyme-Linked Immunosorbent Assay10aFemale10aHumans10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aMale10aMiddle Aged10aSerologic Tests1 aWang L1 aIzumi S1 aHe H1 aFujiwara N1 aSaita N1 aYano I1 aKobayashi K1 aTatsumi N00aSerodiagnosis of Hansen's disease/leprosy by enzyme-linked immunosorbent assay using cord factor (trehalose 6,6'-dimycolate) as an antigen.  a165-740 v683 a<p>IgG and/or IgM antibodies against mycobacterial cord factor (trehalose 6,6'-dimycolate, TDM) in sera of 65 patients of Hansen's disease (21 cases with smear-positive and 44 cases with smear-negative) and 60 healthy individuals were tested by enzyme-linked immunosorbent assay (ELISA) with TDM purified from Mycobacterium tuberculosis H37Rv as an antigen. Of 65 patients with Hansen's disease, 58 cases (89.2%) had positive results (21 samples from 21 patients, 100% with acid-fast bacilli positive in the lesion, and 37 samples from 44 patients, 84.0% with acid-fast bacilli negative Hansen's disease diagnosed clinically). The sensitivity and specificity of anti-cord factor ELISA were higher than those of anti-phenolic glycolipid-I (PGL-I) agglutination test. Among the total, 34 patients were classified clinically into three types of the disease, lepromatous leprosy (LL), borderline lepromatous (BL) and borderline tuberculoid (BT). The antibody titer showed LL > BL > BT, indicating that the elevation of anti-cord factor antibody titers appeared to be parallel with the degree of humoral immune response against M. leprae. By using semisynthetic cord factor consisting of a single subclass of mycolic acid from M. tuberculosis, it was revealed that sera from patients with Hansen's disease were highly reactive against alpha-mycoloyl cord factor (alpha-TDM) and less reactive against methoxy mycoloyl TDM (methoxy TDM), differed from sera of tuberculosis patients, which were highly reactive against both methoxy and alpha-mycoloyl cord factor (alpha-TDM). Most of sera from patients with Hansen's disease were more reactive against TMM than TDM, differed from sera of tuberculosis patients which were highly reactive against TDM. ELISA using TDM as an antigen is simple, reproducible and useful for the rapid serodiagnosis of Hansen's disease, especially for smear-negative cases.</p>  a1342-3681