01931nas a2200409   4500000000100000008004100001260001300042653001500055653001000070653000900080653002200089653002600111653002400137653002300161653001000184653002100194653002000215653003800235653001600273653001100289653001200300653001600312653002400328653002500352653003200377653001700409100001600426700001300442700001200455700001500467245015200482856004100634300001100675490000700686520081400693022001401507       1993                            d  c1993 Dec10aAdolescent10aAdult10aAged10aAged, 80 and over10aAntibodies, Bacterial10aAntigens, Bacterial10aBacterial Proteins10aChild10aChild, Preschool10aCross Reactions10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aleprosy10aMiddle Aged10aMycobacterium bovis10aMycobacterium leprae10aSensitivity and Specificity10aTuberculosis1 aVikerfors T1 aOlcén P1 aWiker H1 aWatson J D00aSerological response in leprosy and tuberculosis patients to the 18-kDa antigen of Mycobacterium leprae and antigen 85B of Mycobacterium bovis BCG.  uhttp://ila.ilsl.br/pdfs/v61n4a06.pdf  a571-800 v613 a<p>Two enzyme-linked immunosorbent assays (ELISAs) for the detection of leprosy antibodies were developed. These assays were based on the recombinant 18-kDa protein of Mycobacterium leprae and the 85B antigen of M. bovis. Sera from leprosy and tuberculosis patients were analyzed, and a phenolic glycolipid-I (PGL-I) ELISA was used as a reference test. The 18-kDa ELISA reached a sensitivity of 70% on lepromatous leprosy sera. The corresponding results for the 85B and the PGL-I ELISAs were 93% and 96%, respectively. The sensitivity on tuberculoid leprosy sera was 72% for the 18-kDa antigen, 95% for the 85B antigen, and 60% for the PGL-I antigen. The 18-kDa antigen ELISA was found to have crossreactivity to sera from patients with tuberculosis, while the 85B and PGL-I assays had high specificity.</p>
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