02187nas a2200337   4500000000100000008004100001260001300042653002600055653002400081653002300105653002400128653001100152653002100163653001200184653002100196653002500217100001400242700002000256700001400276700001900290700001600309700001100325700001300336700001300349245010800362856004100470300001000511490000700521520130700528022001401835       1994                            d  c1994 Mar10aAntibodies, Bacterial10aAntigens, Bacterial10aBacterial Proteins10aHeat-Shock Proteins10aHumans10aImmunoglobulin G10aleprosy10aMolecular Weight10aMycobacterium leprae1 aLaunois P1 aN'Diaye Niang M1 aDrowart A1 aVan Vooren J P1 aSarthou J L1 aLalu T1 aMillan J1 aHuygen K00aIgG response to purified 65- and 70-kDa mycobacterial heat shock proteins and to antigen 85 in leprosy.  uhttp://ila.ilsl.br/pdfs/v62n1a07.pdf  a48-540 v623 a<p>IgG antibody response to mycobacterial heat-shock proteins (hsp) (the 70-kDa antigen from Mycobacterium tuberculosis and M. bovis BCG; the 65-kDa antigen from M. leprae and M. bovis BCG) and to the fibronectin-binding antigen 85 from M. bovis BCG was analyzed in a dot-blot assay in plasma from leprosy patients and their contacts. Most plasma--whatever the status of the subjects--reacted to the hsp 70; 8 of 9 (89%) of paucibacillary patients recognized the 65 mycobacterial hsp but only 2 of 9 (22%) recognized the antigen 85. In contrast, 12 of 12 (100%) of multibacillary patients reacted with the antigen 85 and only 4 of 12 (33%) reacted to the hsp 65 from M. leprae. On the one hand, 7 of 25 (28%) of the lepromin-positive contacts and 2 of 9 (22%) of the lepromin-negative contacts recognized the antigen 85. On the other hand, 11 of 25 (44%) of the lepromin-positive contacts but only 1 of 9 (11%) of the lepromin-negative contacts reacted to the hsp65 from M. leprae. Finally, very few (10%) of the lepromin-positive controls showed a positive reaction to the M. leprae 65-kDa antigen, the BCG 65-kDa antigen, and the 85-kDa antigen of BCG. Thus, differences in binding to the hsp65 from M. leprae and to antigen 85 could be helpful in distinguishing different forms of the disease.</p>
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