01566nas a2200253   4500000000100000008004100001260001300042653002400055653001800079653001100097653002800108653004400136653001600180653001800196100001300214700001300227700001500240700001100255245005500266300001100321490000700332520095900339022001401298       1994                            d  c1994 Jun10aAmino Acid Sequence10aBase Sequence10aHumans10aMolecular Sequence Data10aReceptors, Antigen, T-Cell, gamma-delta10aSarcoidosis10aT-Lymphocytes1 aTamura N1 aSuzuki K1 aYAMAMOTO T1 aKira S00a[The gamma delta T-cell receptors in sarcoidosis].  a1473-90 v523 a<p>T-cells recognize specific antigen (s) through the T-cell antigen receptors (TCR). gamma delta T-cells compose a minor population of the peripheral blood lymphocytes (PBL). These lymphocytes play a central role in recognizing the mycobacterial antigens and granuloma formation in tuberculosis and leprosy. Significant increase of gamma delta T-cells in PBL is observed in a subgroup of sarcoidosis. The majority of gamma delta T-cells showed V gamma 9/V delta 2 recombination, which is common in normal blood. To evaluate the clonality of the increased gamma delta T-cells in PBL, several V gamma 9 and V delta 2 cDNA clones were established. Analysis of V-J junctional sequences of V gamma 9 clones revealed oligoclonal expansion in some cases. Most of V delta 2 clones showed unique sequences. Although no clonal expansion of gamma delta T-cells were observed, TCR-gamma chain might recognize limited numbers of putative antigens in sarcoidosis.</p>  a0047-1852