02016nas a2200325   4500000000100000008004100001260001300042653002600055653002700081653002400108653002200132653002000154653001100174653002100185653002500206653001200231653001800243100001400261700001300275700001400288700001100302700001500313700001900328245007900347300001000426490000700436050001600443520121700459022001401676       1994                            d  c1994 Dec10aAntibodies, Bacterial10aAntibodies, Monoclonal10aAntigens, Bacterial10aBlotting, Western10aCross Reactions10aHumans10aImmunoglobulin G10aIsoelectric Focusing10aleprosy10aMycobacterium1 aDrowart A1 aHuygen K1 aLaunois P1 aJann E1 aNyabenda J1 aVan Vooren J P00aIgG humoral response against the antigen 85 complex homologues in leprosy.  a643-70 v40  aDROWART19943 a<p>Antigen 85 complex is the major protein component present in M. bovis BCG culture filtrate (CF). It consists of a family of three proteins: 85A, 85B and 85C. Combining isoelectric focusing and Western blot analysis, we have previously identified different antigenically related proteins present in the CF of other mycobacteria (M. tuberculosis, M. kansasii, M. avium, M. gordonae, M. fortuitum and M. phlei) using monoclonal antibodies (MoAbs) directed against the antigen 85 complex of M. bovis BCG. Humoral immune response directed against these cross-reactive homologues was analysed in sera from 20 patients with multibacillary leprosy (BL/LL), from 20 patients with paucibacillary leprosy (BT/TT) and from 15 healthy leprosy contacts. All the antigen 85 homologues identified in the seven CFs by MoAbs were also recognized by IgG present in sera from multibacillary leprosy patients, but not or very faintly in sera from paucibacillary leprosy patients or from healthy subjects. These results suggest that some of the M. leprae epitopes inducing a significant humoral response in multibacillary leprosy are common to the various 85 antigenically related proteins present in all mycobacterial species.</p>  a0300-9475