01767nas a2200349   4500000000100000008004100001260001700042653001200059653002400071653001600095653002300111653002000134653001400154653002800168653001600196653001100212653001200223653001100235653002400246653002500270653001200295653001500307653001200322100001700334700001500351245012500366856004100491300001100532490000700543520085300550022001401403       1976                            d  c1976 Jul-Sep10aAnimals10aAntigens, Bacterial10aBCG Vaccine10aCell Fractionation10aCross Reactions10aCytoplasm10aDiagnosis, Differential10aGuinea Pigs10aHumans10aleprosy10aLipase10aMycobacterium bovis10aMycobacterium leprae10aPronase10aSkin Tests10aTrypsin1 aElliston E P1 aTAYLOR C E00aSeparation of M. leprae from human leproma and the development of a cytoplasmic skin test antigen from purified bacilli.  uhttp://ila.ilsl.br/pdfs/v44n3a05.pdf  a319-310 v443 a<p>A successful method for purification of M. leprae from human leproma without subjection to heat has been developed. The "floater" phenomenon has been described which consists of bacillary tendency to float in the supernate when bacilli which are not autoclaved are separated from tissues by enzymatic digestion. A method for preparing cytoplasmic fractions from purified M. leprae has been developed for the production of a skin test antigen for leprosy. The cytoplasmic fraction of M. leprae elicited positive skin test responses in people with tuberculoid leprosy and negative responses in lepromatous leprosy. Cytoplasmic preparations from purified M. leprae had little cross-reactive relationship with the organism BCG. The small particulate fraction elicited positive reactions in PPD-S negative as well as BCG vaccinated individuals.</p>
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