03775nas a2200505   4500000000100000008004100001260001200042653001200054653001800066653001800084653001100102653002700113653002700140653001100167653001900178653001000197653002000207653001100227653001800238653001200256653001400268653000900282653000900291653002800300653001300328653004600341653003600387653002400423653001700447100001300464700001500477700001500492700001400507700001900521700001100540700001200551700001500563700001400578700001300592245017700605300001100782490000700793520245500800022001403255       1993                            d  c1993 1010aAnimals10aB-Lymphocytes10aBase Sequence10aBrazil10aCell Line, Transformed10aDisease Susceptibility10aFemale10aGene Frequency10aGenes10aGenetic Markers10aHumans10aLeishmaniasis10aleprosy10aLod Score10aMale10aMice10aMolecular Sequence Data10aPedigree10aPolymorphism, Restriction Fragment Length10aSequence Homology, Nucleic Acid10aSpecies Specificity10aTuberculosis1 aShaw M A1 aAtkinson S1 aDockrell H1 aHussain R1 aLins-Lainson Z1 aShaw J1 aRamos F1 aSilveira F1 aMehdi S Q1 aKaukab F00aAn RFLP map for 2q33-q37 from multicase mycobacterial and leishmanial disease families: no evidence for an Lsh/Ity/Bcg gene homologue influencing susceptibility to leprosy.  a251-710 v573 a<p>The mycobacterial diseases leprosy and tuberculosis (TB) and the leishmaniases are characterized by a wide spectrum of disease phenotypes, and by the fact that the majority of individuals exposed to the causative organisms Mycobacterium leprae, M. tuberculosis and Leishmania sp. become infected but do not present with clinical disease. In order to determine whether a human homologue to the murine macrophage resistance gene Lsh/Ity/Bcg influences susceptibility to human disease, multicase families for all three diseases have been collected, and linkage analysis performed using a panel of markers in the region of human chromosome 2q33-q37 known to be conserved with the Lsh/Ity/Bcg-containing region of murine chromosome 1. Because of the paucity of available polymorphic markers/linkage information for 2q33-q37, data from 35 multicase leprosy, TB and visceral leishmaniasis families (310 individuals) were first pooled to produce a detailed RFLP map of the region. Peak LOD scores well in excess of 3 were observed for linkage between adjacent pairs of a more proximal (2q33-q35) set of markers CRYGP1, MAP2, FN1, TNP1, VIL1 and DES, and between adjacent pairs of a more distal (2q35-q37) set COL6A3, D2S55 and D2S3. These peak LOD scores and the corresponding values for theta were used in the MAP92 program to generate a multiple two-point map with gene order/map intervals (cM) of: CRYGP1-4.65-MAP2-3.45-FN1-5.95-TNP1-3.41-VIL1-3. 01- DES-20.14-COL6A-10.91-D2S55-3.67-D2S3. Although local support for the placement of loci in this order was weak (LOD < 2, except for DES-COL6A3 where LOD = 6.02), the map is consistent with the gene order for those loci (Cryg, Fn-1, Tp-1, Vil, Des, Col6a3) previously mapped in the mouse. Data from 17 multicase leprosy families (149 individuals) were further analysed for linkage between a putative disease susceptibility locus (DSL) controlling susceptibility to leprosy per se and each of the marker loci. Assuming 100% penetrance for the susceptibility allele, no positive LOD score was obtained for linkage between the DSL and any of the marker genes. Instead, the data provide convincing evidence (LOD scores < -2) that a DSL does not fall within 10-20 cM of CRYGP1, MAP2, TNP1, VIL1, DES or D2S55, or within 5-10 cM of FN1, COL6A3 or D2S3. This effectively excludes a putative DSL controlling susceptibility to leprosy per se from the entire region 2q33-q37.(ABSTRACT TRUNCATED AT 400 WORDS)</p>  a0003-4800