01611nas a2200349   4500000000100000008004100001260001600042653002400058653003800082653001100120653002300131653002100154653001200175653002600187653002500213653001000238653003000248653001800278653001400296653001500310100001300325700001500338700001600353700001500369700001700384245015200401300001100553490000800564050001300572520066200585022001401247       1994                            d  c1994 Nov 1010aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aHumans10aImmunity, Cellular10aInterferon-gamma10aleprosy10aLymphocyte Activation10aMycobacterium leprae10aNepal10aSeroepidemiologic Studies10aT-Lymphocytes10aThymidine10aTuberculin1 aWeir R E1 aMorgan A R1 aBritton W J1 aButlin C R1 aDockrell H M00aDevelopment of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity.  a93-1010 v176  aWEIR19943 a<p>A whole blood assay is described to measure T cell mediated immune responses to leprosy and provide an alternative to the conventional lymphocyte transformation test. Optimal conditions were defined for the whole blood assay, and interferon-gamma measurement was found to be a more sensitive way of measuring responses than tritiated thymidine incorporation. The assay was shown to be useful for investigating responses to a range of leprosy antigens. A whole blood assay has the advantages of being quick, simple and requiring only a small volume of blood, making it more appropriate as an immuno-epidemiological field test in leprosy endemic areas.</p>  a0022-1759