01367nas a2200241   4500000000100000008004100001260001300042653001200055653002300067653001900090653003100109653001700140653001700157100001500174700001500189700001600204700001400220245004800234300001200282490000700294520081000301022001401111       1994                            d  c1994 Dec10aAnimals10aBacterial Vaccines10aDNA, Bacterial10aMycobacterium tuberculosis10aTransfection10aTuberculosis1 aLowrie D B1 aTascon R E1 aColston M J1 aSilva C L00aTowards a DNA vaccine against tuberculosis.  a1537-400 v123 a<p>Expression of the gene for a single mycobacterial antigen (Mycobacterium leprae hsp65) in adult Balb/c mice resulted in substantial cell-mediated protection against challenge with M. tuberculosis. CD4 and CD8 T cells cloned from spleens of such immunized mice passively transferred protection to non-immunized mice, and CD8 cells selectively lysed macrophages infected with M. tuberculosis. Three modes of expressing the gene have been tested: (1) expression from a retroviral vector (pZIPNeoSV) in implanted J774 tumour cells, (2) expression from the same vector via bone marrow cells transfected in vitro and used to reconstitute irradiated mice, and (3) in a preliminary experiment, from CMV immediate-early and hydroxymethylglutaryl Co-A reductase promoters injected as plasmid DNA into muscle.</p>  a0264-410X