02335nas a2200421   4500000000100000008004100001260001300042653001500055653001000070653002400080653001800104653001000122653001100132653001100143653002100154653001800175653001300193653001200206653002600218653000900244653002400253653002500277653001200302653001400314100001400328700001600342700001500358700001100373700001400384700001900398700001600417700001300433245015400446300001000600490000700610520128200617022001401899       1995                            d  c1995 Jan10aAdolescent10aAdult10aAntigens, Bacterial10aChaperonin 1010aChild10aFemale10aHumans10aInterferon-gamma10aInterleukin-210aLepromin10aleprosy10aLymphocyte Activation10aMale10aMycobacterium bovis10aMycobacterium leprae10aSenegal10aTh1 Cells1 aLaunois P1 aN'Diaye M N1 aCartel J L1 aMane I1 aDrowart A1 aVan Vooren J P1 aSarthou J L1 aHuygen K00aFibronectin-binding antigen 85 and the 10-kilodalton GroES-related heat shock protein are the predominant TH-1 response inducers in leprosy contacts.  a88-930 v633 a<p>Peripheral blood mononuclear cells from 27 healthy leprosy contacts were analyzed for lymphoproliferation and TH-1 cytokine secretion (interleukin-2 and gamma interferon) in response to heat shock proteins with molecular masses of 65, 18, and 10 kDa from Mycobacterium leprae and the 30-32-kDa antigen 85 (Ag 85) from Mycobacterium bovis BCG. Cells from 18 and 19 of 19 lepromin-positive contacts proliferated or produced TH-1 cytokines in response to the M. leprae 10-kDa protein and to Ag 85, respectively. Limiting-dilution analysis for two lepromin-positive contacts indicated that about one-third of M. leprae-reactive T cells displayed specificity to the M. leprae 10-kDa protein and Ag 85. The M. leprae 65- and 18-kDa proteins were less potent TH-1 response inducers: gamma interferon and interleukin-2 could be measured in 14 and 19 lepromin-positive contacts, respectively. In contrast, very low or undetectable proliferative and cytokine responses were found for 8 lepromin-negative contacts. Our data demonstrate that the fibronectin-binding Ag 85 and the 10-kDa GroES homolog are powerful mycobacterial TH-1 response inducers in the vast majority of lepromin-positive contacts and suggest that they might be valuable candidates for a future subunit vaccine.</p>  a0019-9567