01742nas a2200253   4500000000100000008004100001260001300042653002800055653001100083653002000094653001200114653002400126653002600150100001300176700001400189700001100203700001200214700001200226245008600238300001000324490000700334520113300341022001401474       1993                            d  c1993 Mar10aDiagnosis, Differential10aHumans10aInfant, Newborn10aleprosy10aLipopolysaccharides10aStaining and Labeling1 aButt K I1 aKawatsu K1 aWang T1 aMaeda Y1 aIzumi S00aImmunopathological stain of lipoarabinomannan-B (LAM-B) for diagnosis of leprosy.  a13-200 v623 a<p>We developed an immunopathological staining of LAM-B antigen in formalin-fixed paraffin-embedded tissues, and compared it with, PGL-I immunostaining, Fite Faraco's stain and periodic acid carbol pararosaniline (PACPR) stain. Out of the total 28 leprosy cases, 27 were positive to LAM-B immunostaining while 23 were positive to PGL-I stain. Fite's stain was positive in 21 cases while PACPR stain was positive in 24 cases. In scrofuloderma, LAM-B antigen was observed only in the granuloma while no other positive findings were noted with other stains. Normal skin did not give any positive findings with any of the stains. Other dermatoses showed no positive findings to any of the stains tested. LAM-B staining was observed in the nerve even in the absence of bacilli in leprosy tissues. Presence of LAM-B in the cutaneous nerves is helpful in discriminating leprosy from other mycobacterioses. Considering the high sensitivity of LAM-B and the predilection of M. leprae for the nerves, we concluded that LAM-B staining can be a useful new tool in the prompt diagnosis of leprosy, especially in suspected or early cases.</p>  a0386-3980