02738nas a2200361   4500000000100000008004100001260001300042653001400055653002000069653001400089653001500103653001600118653002300134653001700157653001100174653001800185653001800203653002100221653002900242653001200271653001700283653000900300653003200309100001400341700001500355700001800370700001300388245015000401300001100551490000700562520179300569022001402362       1994                            d  c1994 Nov10aChromatin10aChronic Disease10aCytoplasm10aEpithelium10aFibroblasts10aGranuloma Annulare10aHidradenitis10aHumans10aInterleukin-110aInterleukin-610aLangerhans Cells10aLeishmaniasis, Cutaneous10aleprosy10aPlasma Cells10aSkin10aTumor Necrosis Factor-alpha1 aAhmed A A1 aNordlind K1 aSchultzberg M1 aLidén S00aInterleukin-1 alpha- and beta-, interleukin-6- and tumour necrosis factor-alpha-like immunoreactivities in chronic granulomatous skin conditions.  a435-400 v743 a<p>Paraformaldehyde-fixed tissue of chronic granulomatous skin conditions, such as cutaneous leishmaniasis, granuloma annulare, leprosy and hidroadenitis, was investigated for the presence of interleukin-1 alpha-, interleukin-1 beta-, interleukin-6- and tumour necrosis factor-alpha-like immunoreactivities among the cellular infiltrates. There was a weak to strong cytoplasmic labelling of plasma cells for interleukin-6 and tumour necrosis factor-alpha at the periphery of the granulomatous mass and around the skin appendages. The interleukin-6-like immunoreactivity seemed to be correlated with the coarseness of the chromatin material of the cells, being more intense with coarse chromatin. The cytoplasmic labelling for interleukin-1 alpha and interleukin-1 beta in the plasma cells was less intense. Epitheloid, Langhans' giant cells and small round cells exhibited a weak to moderate cytoplasmic labelling for interleukin-1 alpha and interleukin-1 beta, whereas the staining intensity for interleukin-6 and tumour necrosis factor-alpha was weak to strong. In addition, there was staining of the stroma in the centre of granuloma with antisera against interleukin-1 alpha, interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha. This area contained few cells, suggesting that the granuloma was in a resolution process. A contribution of interleukin-6 and tumour necrosis factor-alpha to the granulomatous reaction, at least during the maintenance period, is suggested by the occurrence of these cytokines in the skin conditions studied. The findings are also consistent with a suggested role of B cells in the late stages of the granulomatous reaction. In addition, they are in line with the reported declining role of interleukin-1 in the maintenance of granuloma.</p>  a0001-5555