02141nas a2200313   4500000000100000008004100001260001300042653003200055653003000087653002600117653003800143653001800181653001100199653001200210100001300222700001400235700001400249700001200263700001200275700001900287700001400306700001300320700001600333245015900349300001100508490000600519520128800525022001401813       1994                            d  c1994 Dec10aAntibodies, Anticardiolipin10aAntiphospholipid Syndrome10aBeta 2-Glycoprotein I10aEnzyme-Linked Immunosorbent Assay10aGlycoproteins10aHumans10aleprosy1 aHojnik M1 aGilburd B1 aZiporen L1 aBlank M1 aTomer Y1 aScheinberg M A1 aTincani A1 aRozman B1 aShoenfeld Y00aAnticardiolipin antibodies in infections are heterogenous in their dependency on beta 2-glycoprotein I: analysis of anticardiolipin antibodies in leprosy.  a515-210 v33 a<p>We studied the effect of beta 2-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine beta 2-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without beta 2-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of beta 2-GPI on the leprosy and APS sera binding to CL was confirmed using purified human beta 2-GPI. Enhanced binding was seen if beta 2-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against beta 2-GPI were found by ELISA. Leprosy anti-beta 2-GPI antibodies appear to be a separate antibody population recognizing only beta 2-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their beta 2-GPI requirement for binding to CL.</p>  a0961-2033