01904nas a2200385   4500000000100000008004100001260001300042653001800055653001500073653001900088653003000107653002900137653002100166653001100187653002300198653001200221653002800233653002500261653003000286653003000316653003200346653000900378100001200387700001300399700001400412700001600426700001600442700001100458245012800469856004100597300001000638490000700648520084900655022001401504       1995                            d  c1995 Mar10aBase Sequence10aDNA Probes10aDNA, Bacterial10aElectrophoresis, Agar Gel10aFalse Negative Reactions10aGenes, Bacterial10aHumans10aLeprostatic Agents10aleprosy10aMolecular Sequence Data10aMycobacterium leprae10apolymerase chain reaction10aPredictive Value of Tests10aSensitivity and Specificity10aSkin1 aMisra N1 aRamesh V1 aMisra R S1 aNarayan N P1 aColston M J1 aNath I00aClinical utility of LSR/A15 gene for Mycobacterium leprae detection in leprosy tissues using the polymerase chain reaction.  uhttp://ila.ilsl.br/pdfs/v63n1a06.pdf  a35-410 v633 a<p>Skin biopsy and slit-skin smears from 46 leprosy patients and 4 nonleprosy patients were tested for the presence of Mycobacterium leprae by the polymerase chain reaction (PCR) using primers based on the sequence of the LSR/15 kD gene. The PCR was found to be specific and sensitive, with a detection level of 10 and 100 bacilli. PCR using skin biopsies gave a higher detection rate than did slit-skin smears, probably due to the higher density of bacilli in a 4-mm punch biopsy. Dot blot hybridization with radioactive probes was 10-fold more sensitive than the ethidium bromide staining. Eight patients who did not show acid-fast bacilli in tissues by the conventional methods were shown to have PCR-amplified M. leprae DNA. False-negative results were obtained in 3 cases even though formal evidence for tissue inhibitors was absent.</p>
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