02428nas a2200409   4500000000100000008004100001260001300042653001200055653002400067653002000091653002700111653003800138653001600176653002100192653002100213653001200234653002400246653002500270653002500295100001500320700000900335700001200344700001500356700001300371700001500384700001800399700001300417700001800430700001500448245010600463856005900569300001100628490000700639050003200646520132600678022001402004       1995                            d  c1995 Jun10aAnimals10aAntigens, Bacterial10aCercocebus atys10aDisease Models, Animal10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aImmunoglobulin G10aImmunoglobulin M10aleprosy10aLipopolysaccharides10aLongitudinal studies10aMycobacterium leprae1 aGormus B J1 aXu K1 aCho S N1 aBaskin G B1 aBohm R P1 aMartin L N1 aBlanchard J L1 aMack P A1 aRatterree M S1 aMeyers W M00aExperimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys.  uhttp://leprev.ilsl.br/pdfs/1995/v66n2/pdf/v66n2a03.pdf  a105-250 v66  aInfolep Library - available3 a<p>In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin Mycobacterium leprae (ML) between 4.5 x 10(8) and 1 x 10(9) by either combined IV/IC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of 1 x 10(9) ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority of leprosy-susceptible SMM in contrast to negative levels in more resistant animals. Anti-LAM IgG OD values exceeded the positive cut-off point after inoculation in 5 of 11 SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen.</p>  a0305-7518