02295nas a2200445 4500000000100000008004100001260001700042653001500059653001000074653001100084653003000095653001000125653002100135653001100156653001900167653003800186653001300224653001100237653001100248653002000259653002800279653002800307653000900335653001600344653003000360653002600390653004600416653001600462100001700478700001300495700001300508700001500521700001700536245013600553856004800689300001000737490000700747520108100754022001401835 2010 d c2010 May-Jun 10 aAdolescent 10 aAdult 10 aBrazil 10 aCation Transport Proteins 10 aChild 10 aChild, Preschool 10 aFemale 10 aGene Frequency 10 aGenetic Predisposition to Disease 10 agenotype 10 aHumans 10 aInfant 10 aInfant, Newborn 10 aLeprosy, Multibacillary 10 aLeprosy, Paucibacillary 10 aMale 10 aMiddle Aged 10 apolymerase chain reaction 10 aPolymorphism, Genetic 10 aPolymorphism, Restriction Fragment Length 10 aYoung Adult 1 aTeixeira MAG 1 aSilva NL 1 aRamos AL 1 aHatagima A 1 aMagalhães V 00 a[NRAMP1 gene polymorphisms in individuals with leprosy reactions attended at two reference centers in Recife, northeastern Brazil]. uhttp://www.scielo.br/pdf/rsbmt/v43n3/14.pdf a281-6 0 v43 3 aINTRODUCTION: To investigate susceptibility to leprosy reactions, three polymorphisms of the natural resistance-associated macrophage protein (NRAMP1) gene were determined in 201 individuals who were attended at two reference centers in Recife, between 2007 and 2008. Of these, 100 were paucibacillary and 101 were multibacillary.
METHODS: The 274C/T, D543N and 1729+55del4 polymorphisms of the NRAMP1 gene were determined using the technique of restriction fragment polymorphism on DNA extracted from peripheral blood. Allelic and genotypic frequencies were estimated by direct counting.
RESULTS: The predominant genotypes were: CC (51.8%) for 274C/T; GG (86.6%) for D543N; and +-TGTG (59.9%) for 1729+55del4. The mutant genotype 274 TT predominated in negativity of the reverse reaction (p = 0.03) and in positivity of erythema nodosum leprosum (p = 0.04).
CONCLUSIONS: Our results suggest that 274 C/T polymorphism of the NRAMP1 gene may aid in determining the susceptibility to type II reactions among leprosy patients.
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