01839nas a2200385   4500000000100000008004100001260001700042653001500059653001000074653000900084653002600093653002400119653003000143653003800173653001100211653001600222653001100238653001200249653001800261653000900279653001600288653002500304100002500329700001500354700001400369700001400383700001200397700001300409245011300422856005500535300001100590490000700601520083100608022001401439       1999                            d  c1999 Jul-Aug10aAdolescent10aAdult10aAged10aAntibodies, Bacterial10aAntigens, Bacterial10aBlood Specimen Collection10aEnzyme-Linked Immunosorbent Assay10aFemale10aGlycolipids10aHumans10aleprosy10aLinear Models10aMale10aMiddle Aged10aMycobacterium leprae1 aTomimori-Yamashita J1 aNguyen T H1 aMaeda S M1 aFlageul B1 aRotta O1 aCruaud P00aAnti-phenolic glycolipid-I (PGL-I) determination using blood collection on filter paper in leprosy patients.  uhttp://www.scielo.br/pdf/rimtsp/v41n4/v41n4a08.pdf  a239-420 v413 a<p>The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals.</p>  a0036-4665