01077nas a2200265 4500000000100000008004100001260001300042653001300055653001600068653001100084653003000095653001300125653001200138100001500150700001200165700001100177700001600188700001600204245015100220300001100371490000700382050003200389520037600421022001400797 1982 d c1982 Apr10aAntigens10aBCG Vaccine10aHumans10aHypersensitivity, Delayed10aLepromin10aleprosy1 aSengupta U1 aSinha S1 aRamu G1 aMukherjee A1 aDesikan K V00aAssessment of Dharmendra antigen. (V) attempts for purification of specific skin delayed hypersensitivity (DH) inducing antigen (S) from lepromin. a208-130 v54 aInfolep Library - available3 a

After electrophoresis (in pyrogen-free conditions) of Dharmendra antigen sonicates on agarose gel slides, the components (anionic and cationic) were eluted in pyrogen-free normal saline. On skin testing, it was found that only anionic component produced specific skin DH reactions in tuberculoid leprosy patients. The significance of the observations is discussed.

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