02196nas a2200289   4500000000100000008004100001260001300042653002100055653001800076653001600094653001200110653003000122653001100152653001200163653001600175653001600191653001700207100001700224700001500241700001500256700001500271245015800286300001000444490000700454520143100461022001401892       1982                            d  c1982 May10aCandida albicans10aCell Movement10aClofazimine10aDapsone10aDrug Therapy, Combination10aHumans10aleprosy10aLymphocytes10aNeutrophils10aPhagocytosis1 aRensburg C E1 aGatner E M1 aImkamp F M1 aAnderson R00aEffects of clofazimine alone or combined with dapsone on neutrophil and lymphocyte functions in normal individuals and patients with lepromatous leprosy.  a693-70 v213 a<p>The effects of clofazimine on neutrophil activities such as random motility, migration to the leukoattractants endotoxin-activated serum and N-formyl-L-methionyl-L-leucyl-L-phenylalanine phagocytosis of Candida albicans, postphagocytic hexose-monophosphate shunt activity, and myeloperoxidase-mediated iodination and the effects of clofazimine on lymphocyte transformation to mitogens were assessed in vitro and after ingestion of the drug by normal individuals and patients with lepromatous leprosy. For in vitro studies, the concentration range of the drug investigated was 10(-6) M to 10(-2) M. for in vivo studies, subjects ingested 200 mg of clofazimine daily for a period of 5 days. At concentrations of 5 X 10(-6) M to 5 X 10(-3) M clofazimine caused a progressive dose-dependent inhibition of neutrophil motility without detectable effects on phagocytosis, postphagocytic hexose-monophosphate shunt activity, or myeloperoxidase-mediated iodination. Over the same concentration range, clofazimine inhibited lymphocyte transformation. The inhibitory effect on neutrophil motility was associated with a spontaneous stimulation of oxidative metabolism and could be prevented by coincubation of dapsone with clofazimine. after ingestion of clofazimine responsiveness of lymphocytes to mitogens was decreased in normal volunteers and leprosy patients: neutrophil motility in normal individuals was likewise inhibited.</p>  a0066-4804