02021nas a2200301   4500000000100000008004100001260001300042653001200055653001500067653001100082653002000093653003100113653001500144653001500159653000900174653000900183653003100192653002500223653001800248100001800266700001500284700001400299245006700313300001100380490000700391520130700398022001401705       1980                            d  c1980 Dec10aAnimals10aDetergents10aFemale10aHot Temperature10aHydrogen-Ion Concentration10aHydrolases10aHydrolysis10aIron10aMice10aMycobacterium lepraemurium10aPhosphatidylcholines10aPhospholipids1 aKashiwabara Y1 aNakagawa H1 aMatsuki G00aPhospholipid deacylating activities in murine leprosy bacilli.  a1861-80 v883 a<p>1) The particulate fraction of cultivated murine leprosy bacilli (Mycobacterium lepraemurium, rough colonies of the Hawaiian-Ogawa strain) contained phospholipid deacylating activities with acidic pH optima. It hydrolyzed phosphatidylcholine and phosphatidylethanolamine at similar rates, and phosphatidylinositol oligomannosides more slowly. It also hydrolyzed 1-acyl- and 2-acyl-GPCs (sn-glycerol 3-phosphocholine) more rapidly than phosphatidylcholine. 2) Ca2+ did not stimulate either diacyl- or monoacyl-hydrolase activity. Triton X-100 and Emulgen 913 had little influence on the hydrolysis of phosphatidylcholine, but at rather high concentrations inhibited the hydrolyses of 1-acyl- and 2-acyl-GPCs. Iron ions strongly inhibited the hydrolysis of phosphatidylcholine, but caused little or no inhibition of the deacylations of 1-acyl- and 2-acyl-GPCs. 3) With 1-[stearoyl-14C]phosphatidylcholine and 2-[oleoyl-14C]phosphatidylcholine as substrates, both labeled fatty acid and lysophosphatidylcholine were produced. Labeled fatty acid appeared more rapidly from 2-[oleoyl-14C]phosphatidylcholine than labeled lysophosphatidylcholine, while labeled lysophosphatidylcholine was produced more than labeled fatty acid from 1-[stearoyl-14C]phosphatidylcholine in the early stage of incubation.</p>  a0021-924X