01275nas a2200289 4500000000100000008004100001260001300042653001500055653001700070653001100087653001200098653001800110653002500128653002500153653001800178100001200196700001600208700001600224700001100240700001600251245007100267856004100338300001100379490000700390520057400397022001400971 1980 d c1980 Dec10aAbsorption10aEpoxy Resins10aHumans10aleprosy10aMethacrylates10aMicroscopy, Electron10aMycobacterium leprae10aResins, Plant1 aOKADA S1 aFukunishi Y1 aMukherjee A1 aRamu G1 aDesikan K V00aAn improved embedding method for electron microscopy of lepromata. uhttp://ila.ilsl.br/pdfs/v48n4a06.pdf a408-130 v483 a
Spurr's resin mixture has been found to be the embedding material most suitable for electron microscopic studies of lepromata at present. Like methacrylate, it can penetrate well into foamy structures and the leprosy bacilli within foamy structures. On the other hand, like Epon 812, it is stable against the electron beam and can preserve ultrafine structures. Additionally, we have found that the use of dimethylformamide or dimethylsulfoxide instead of propylene oxide as substituter improves the preservation of the ultrafine structures of leprosy bacilli.
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