01848nas a2200421   4500000000100000008004100001260001300042653002700055653002400082653002300106653001500129653001900144653001800163653001600181653002300197653001100220653001600231653001100247653002500258653001200283653002400295653001600319653000900335653002200344653003200366653000900398100002000407700002200427700001200449700001600461245013800477856005900615300001100674490000700685050003200692520068800724022001401412       1999                            d  c1999 Sep10aAntibodies, Monoclonal10aAntigens, Bacterial10aBacterial Proteins10aBiomarkers10aBiopsy, Needle10aChaperonin 6010aChaperonins10aCulture Techniques10aFemale10aGlycolipids10aHumans10aImmunohistochemistry10aleprosy10aLipopolysaccharides10aMacrophages10aMale10aPeripheral nerves10aSensitivity and Specificity10aSkin1 aVan den Bos I C1 aKhanolkar-Young S1 aDas P K1 aLockwood DN00aImmunohistochemical detection of PGL-1, LAM, 30 kD and 65 kD antigens in leprosy infected paraffin preserved skin and nerve sections.  uhttp://leprev.ilsl.br/pdfs/1999/v70n3/pdf/v70n3a07.pdf  a272-800 v70  aInfolep Library - available3 a<p>A panel of lipid, carbohydrate and protein antibodies were optimized for use in detecting M. leprae antigens in paraffin embedded material. Skin and nerve biopsies from 13 patients across the leprosy spectrum were studied. All antibodies detected antigen in tissues with a BI > 1. Phenolic-glycolipid was not detected in bacteriologically negative tissue but lipoarabinomanan (LAM) and protein antigens were detected. Staining with LAM was strongest and gave least background. The transfer of this immunohistochemical technique to paraffin embedded material will allow examination of tissue with better morphology and from clinics without access to tissue freezing facilities.</p>  a0305-7518