01299nas a2200277   4500000000100000008004100001260001300042653002600055653001100081653002300092653001100115653002100126653001200147653002500159653000900184100001800193700001900211700001200230700001300242245010700255856007800362300001200440490000700452520054800459022001401007       1980                            d  c1980 Feb10aAntibodies, Bacterial10aBiopsy10aCulture Techniques10aHumans10aImmunoglobulin G10aleprosy10aMycobacterium leprae10aSkin1 aLai A Fat R F1 aChan Pin Jin J1 aFurth R1 aHarboe M00aIn vitro synthesis of anti-mycobacterial antibodies in biopsies from skin lesions of leprosy patients.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC550764/pdf/iai00170-0015.pdf  a297-3010 v273 a<p>To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The results show that anti-M. leprae antibodies were synthesized in vitro in the biopsies from the skin lesions of leprosy patients and that the specificity of the locally produced antibodies varied from patient to patient.</p>  a0019-9567