01197nas a2200265   4500000000100000008004100001260001700042653001200059653001000071653001100081653001200092653001200104653000900116653001800125653000900143653002000152653002600172100001300198245008700211856004100298300001000339490000700349520056100356022001400917       1977                            d  c1977 Jan-Mar10aAnimals10aColor10aHumans10aleprosy10aMethods10aMice10aMycobacterium10aRats10aRosaniline Dyes10aStaining and Labeling1 aHarada K00aA modified allochrome procedure for demonstrating mycobacteria in tissue sections.  uhttp://ila.ilsl.br/pdfs/v45n1a07.pdf  a49-510 v453 a<p>A modified allochrome staining procedure is presented as being the most reliable and sensitive method for demonstrating mycobacteria in tissue sections. The technic is as follows: Deparaffinize formalin fixed sections, oxidize in 10% periodic acid for 24 hours, differentiate in 1% HCl-70% ethanol, stain in Weigert's iron hematoxylin nuclear stain, and counterstain in picro-methyl glue. Mycobacteria stained brilliant red in contrast with the allochrome-stained background tissues, and apparently otherwise chromophobic bacilli are demonstrated.</p>
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