02048nas a2200361   4500000000100000008004100001260001300042653002100055653002500076653001200101653001800113653001500131653002300146653001100169653001800180653001200198653001100210653001400221653001600235653000900251653000900260653002500269653001700294100002100311700002200332700002100354700002000375245017900395300001100574490000700585520108000592022001401672       1982                            d  c1982 Sep10aAcid Phosphatase10aAlkaline Phosphatase10aAnimals10aAscitic Fluid10aCathepsins10aDeoxyribonucleases10aFemale10aGlucuronidase10aleprosy10aLipase10aLysosomes10aMacrophages10aMale10aMice10aMice, Inbred Strains10aPhagocytosis1 aRojas-Espinosa O1 aRodríguez-Paez L1 aGonzález-Cruz O1 aEstrada-Parra S00aPhagocytosis in leprosy. 5. The effect of the infection with Mycobacterium lepraemurium on the level of diverse hydrolytic lysosomal enzymes of murine peritoneal macrophages.  a306-150 v503 a<p>The effect of the infection with M. lepraemurium on the activity of several lysosomal enzymes of mouse peritoneal cells was studied. The enzymes studies were acid- and alkaline-phosphatases, acid (cathepsin D-type) proteinase, beta-glucuronidase, deoxyribonuclease, a nonspecific lipase, and lysozyme. Enzyme determinations were carried out four months and six months after the infection with 15.5 X 10(7) bacilli per mouse. Clear differences between M. lepraemurium-infected and normal animals were observed at four months of infection, with all of the mentioned enzyme activities well above the normal values. At six months of infection, a tendency to decrease to normal values of the enzyme activities was observed. It is suggested that this biochemical activation of mouse peritoneal cells reflects the effect of the cell-mediated immune response triggered by the infection with the murine leprosy bacillus. M. lepraemurium-infected mice possess macrophages in a high state of biochemical activation; yet, they are unable to get rid of the infecting microorganism.</p>  a0148-916X