01766nas a2200361   4500000000100000008004100001260001300042653001500055653001000070653000900080653002600089653002400115653001600139653001000155653003800165653001100203653001100214653001200225653000900237653001200246653001600258653001800274653002000292653002800312100001500340700001800355700001700373245018300390300001100573490000700584520079900591022001401390       1983                            d  c1983 May10aAdolescent10aAdult10aAged10aAntibodies, Bacterial10aAntigens, Bacterial10aBCG Vaccine10aChild10aEnzyme-Linked Immunosorbent Assay10aFemale10aHumans10aleprosy10aMale10aMannans10aMiddle Aged10aMycobacterium10aPolysaccharides10aTuberculosis, Pulmonary1 aMiller R A1 aDissanayake S1 aBuchanan T M00aDevelopment of an enzyme-linked immunosorbent assay using arabinomannan from Mycobacterium smegmatis: a potentially useful screening test for the diagnosis of incubating leprosy.  a555-640 v323 a<p>A carbohydrate antigen composed predominantly of arabinomannan has been purified from Mycobacterium smegmatis and used in an enzyme-linked immunosorbent assay to detect anti-mycobacterial antibodies in human sera. Sera from 117 controls, 25 tuberculosis patients, 124 leprosy patients and 256 household contacts of leprosy patients were tested. When compared with the control group, 56% of tuberculosis patients, 27% of patients with tuberculoid leprosy, 77% of borderline leprosy cases, and 95% of patients with lepromatous leprosy had elevated titers. Nine percent of the household contact group had abnormally high levels of antibody. The relevance of these findings to the serodiagnosis of incubating leprosy and the management of household contacts of leprosy patients is discussed.</p>  a0002-9637