01853nas a2200265   4500000000100000008004100001260001300042653002600055653002300081653002400104653002000128653001100148653002000159653001200179653002500191653003100216653002800247100001500275700001400290245018400304300001100488490000800499520106600507022001401573       1980                            d  c1980 Dec10aAntibodies, Bacterial10aAntibody Formation10aAntigens, Bacterial10aCross Reactions10aHumans10aImmunodiffusion10aleprosy10aMycobacterium leprae10aMycobacterium tuberculosis10aTuberculosis, Pulmonary1 aKaplan M H1 aChase M W00aAntibodies to mycobacteria in human tuberculosis. II. Response to nine defined mycobacterial antigens with evidence for an antibody common to tuberculosis and lepromatous leprosy.  a835-430 v1423 a<p>Antibodies to mycobacterial antigens were found in the sera of 33 of 52 patients with active tuberculosis by microimmunodiffusion tests. The highest titered sera were examined by a technique in which sera are placed in an intermediate gel between a reference goat antiserum field and a gel containing the antigens separated by one-dimensional electrophoresis. Special patterns caused by the presence of patient's serum during the two-dimensional electrophoresis showed that nine distinct antibodies could be designated by anodal migration of the corresponding nine antigens and the band position with respect to the reference pattern. Six of these antibodies were detected only by sera from selected patients, while the other three antibodies, "Lep," "Da," and "USJ 6," were also detected by the goat antiserum. Lep is present in patients with lepromatous leprosy but had never been described in those with tuberculosis. Monospecific human antisera were used to detect Lep and Da, and new antibody, during fractionation of mycobacterial culture filtrate.</p>  a0022-1899