02060nas a2200337   4500000000100000008004100001260001300042653001000055653001800065653002000083653001100103653003100114653001100145653001300156653001200169653002000181653000900201653002200210653001800232100001600250700001300266700001500279700001300294700002100307700001700328245008700345300001100432490000700443520125800450022001401708       1982                            d  c1982 Jul10aAdult10aB-Lymphocytes10aCells, Cultured10aFemale10aHemolytic Plaque Technique10aHumans10aKinetics10aleprosy10aLeukocyte Count10aMale10aPokeweed Mitogens10aT-Lymphocytes1 aBullock W E1 aWatson S1 aNelson K E1 aSchauf V1 aMakonkawkeyoon S1 aJacobson R R00aAberrant immunoregulatory control of B lymphocyte function in lepromatous leprosy.  a105-140 v493 a<p>The capacity of peripheral blood mononuclear (PBM) cells from patients with leprosy to generate immunoglobulin-secreting cells in response to pokeweed mitogen (PWM) was evaluated by a reverse haemolytic plaque forming cell (PFC) assay. The PFC responses of PBM cells from patients with lepromatous (Lpr) leprosy were significantly higher (P less than 0.01) than those of PBM cells from normal controls and patients with tuberculoid leprosy. Co-culture of T lymphocytes from normal donors with PBM cells from Lpr patients reduced the PFC response of these cells to the normal range. T4+-helper lymphocytes from Lpr donors did not induce supranormal responses to PWM by normal PBM cells enriched for B lymphocytes. T8+-suppressor lymphocytes from normal donors greatly reduced the response of cultures containing normal allogeneic B cells plus T4+ cells. Conversely, when T8+ cells from Lpr donors were cocultured with normal B cells plus T4+ cells, they failed to suppress the response to PWM. In summary, these studies have demonstrated abnormally high PWM-stimulated PFC responses by B lymphocytes from patients with Lpr leprosy. This aberration, in turn, is associated with a loss of regulatory function by T8+-suppressor cells in Lpr patients.</p>  a0009-9104