02125nas a2200253   4500000000100000008004100001260001300042653001200055653001200067653001200079653001500091653001800106653002300124653001100147653001200158653001800170100001100188245013600199856004100335300001000376490000700386520146400393022001401857       1983                            d  c1983 Mar10aAcetone10aAlkanes10aAnimals10aArmadillos10aCulture Media10aDimethyl Sulfoxide10aHumans10aleprosy10aMycobacterium1 aKato L00aIn vitro cultivation of Mycobacterium X from Mycobacterium leprae-infected tissues in acetone-dimethylsulfoxide-tetradecane medium.  uhttp://ila.ilsl.br/pdfs/v51n1a12.pdf  a77-830 v513 a<p>Several strains of mycobacteria were cultivable from Mycobacterium leprae-infected human and armadillo tissues in a liquid medium containing three dimethyl analogs: dimethylketone, dimethylsulfoxide, and tetradecane [CH3 . (CH2)12 . CH3]. The medium contained KH2PO4, 7.0 g; Na2HPO4, 1.0 g; (NH4)2SO4, 2.0 g; MgSO4, 0.1 g; iron ammonium citrate, 0.1 g; DMSO, 10 ml; and acetone, 150 ml in distilled water ad one liter. Tetradecane 0.1 ml was added aseptically to each tube, containing 10 ml of the sterile medium. The media, inoculated with M. leprae were incubated at 38 degrees C and shaken vigorously twice weekly. Growth developed as a fine emulsion at the upper phase of the two-phase system. This was homogenized by mechanical shaking, permitting growth estimation by turbidity measurements. Microscopic examination showed unmistakably the slow but abundant multiplication of acid-fast rods. The logarithmic growth rate was measurable during two to three months, followed by a plateau. The strains are maintained in subcultures by regular transfer into the same medium at two- to three-month intervals. The cultures and subcultures do not grow on Löwenstein or in Dubos media, but in the foot pads of mice they produce a multiplication similar to that obtained following injection of host-grown M. leprae. The cultures are tentatively designated as Mycobacterium X. The relationship of Mycobacterium X to the pathology of leprosy is not clear.</p>
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