01783nas a2200325   4500000000100000008004100001260001300042653001800055653001600073653001900089653001100108653002500119653002800144653001800172653003000190653001900220653002400239653002300263653002700286653003600313100001200349700001300361700001500374245013700389856004100526300000900567490000700576520086000583022001401443       1999                            d  c1999 Mar10aBase Sequence10aDNA Primers10aDNA, Bacterial10aHumans10aLeprosy, lepromatous10aMolecular Sequence Data10aMycobacterium10apolymerase chain reaction10aRNA, Bacterial10aRNA, Ribosomal, 16S10aSequence Alignment10aSequence Analysis, DNA10aSequence Homology, Nucleic Acid1 aSakai T1 aMatsuo E1 aWakizaka A00aComplete DNA sequence analysis for 16S ribosomal RNA gene of the leproma-derived, cultivable and nerve-invading mycobacterium HI-75.  uhttp://ila.ilsl.br/pdfs/v67n1a08.pdf  a52-90 v673 a<p>The complete 1493 nucleotide sequence of the 16SrRNA gene of the leproma-derived and cultivable mycobacterium HI-75 strain was analyzed to elucidate the taxonomic characteristics by direct sequencing of the polymerase chain reaction (PCR) products. The results revealed that the sequence of mycobacterium HI-75 was mostly similar to that of Mycobacterium scrofulaceum with 5 bases differences in the sequenced 1493 bases (0.35%) of the 16SrRNA gene. M. leprae differed from the strain with 47 bases (3.3%). Sasaki and Hamit reported the nerve-invasive activity of the inoculated mycobacterium HI-75 in nude mice or the 131I-treated immunocompromised Swiss mice. The results indicate that mycobacterium HI-75 could be a mutant of M. scrofulaceum possessing the ability to invade the peripheral nerve in addition to developing leproma-like lesions.</p>
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