01539nas a2200361   4500000000100000008004100001260001300042653001000055653000900065653001100074653001400085653002300099653001100122653001200133653001600145653001600161653000900177653001600186653002700202653000900229653001800238100001400256700001300270700001400283700001500297700001300312700001500325245014900340300001100489490000800500520065500508022001401163       1983                            d  c1983 Mar10aAdult10aAged10aFemale10aGranuloma10aHistocytochemistry10aHumans10aleprosy10aLymphocytes10aMacrophages10aMale10aMiddle Aged10aNaphthol AS D Esterase10aSkin10aT-Lymphocytes1 aGaulier A1 aPrat J J1 aWallach D1 aPalangie A1 aLe Sec G1 aCottenot F00aDemonstration of T lymphocytes in leprous granuloma using the acid alpha naphtyl acetate esterase activity. An attempt at quantitative analysis.  a103-140 v1763 a<p>In nine leprosy patients (1 TT, 1 BT, 4 BL and 3 LLp), esterase positive lymphocytes (T Lymphocytes) were studied in frozen sections of skin biopsies by alpha naphtyl acetate esterase pH 5.8 method (ANAE) Four patients had never been treated previously and five patients exhibited clinical and bacteriological evidence of relapse for inadequate therapy at first biopsy. There was an increase in ANAE (+) lymphocyte density in granulomas when second biopsies were done after efficient treatment, evaluated by bacillary index for the eight bacilliferous patients, and clinical improvement. The significance of T cells in granulomas is discussed.</p>  a0344-0338