01843nas a2200229   4500000000100000008004100001260001300042653002400055653004000079653001100119653002800130653001200158653002500170100001600195700001300211700001600224245017100240300001100411490000700422520117000429022001401599       1984                            d  c1984 Jun10aAntigens, Bacterial10aElectrophoresis, Polyacrylamide Gel10aHumans10aImmunoenzyme Techniques10aleprosy10aMycobacterium leprae1 aKlatser P R1 aRens M M1 aEggelte T A00aImmunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera.  a537-440 v563 a<p>In this study the SDS-polyacrylamide gel electrophoresis immunoperoxidase (SGIP) assay was used for characterizing the antigenic components of Mycobacterium leprae using patients' sera. This technique involved the separation of mycobacterial sonicates on SDS-polyacrylamide gels, longitudinal sectioning of the gels, incubation with patients' sera and visualization of the antigen-antibody complexes by the indirect immunoperoxidase technique. A number of antigens present in M. leprae sonicates were recognized by leprosy patients' sera, some of which were seen in other mycobacteria as well. Antibody binding to a 33 kD antigen, present in both M. leprae and BCG sonicates, was reduced only in the latter after 6 months of multiple drug treatment of one patient. It is suggested that this is a common mycobacterial antigen with one or more M. leprae specific determinants. Several antigens were identified only in M. leprae sonicates, only by leprosy patients: a 12, 22, 28, 36, 41 and 86 kD component. These antigens lost their antigenicity after trypsin treatment, but were heat stable. Such M. leprae specific antigens may be useful for immunodiagnosis.</p>  a0009-9104