01772nas a2200301   4500000000100000008004100001260001700042653001200059653001900071653001100090653002300101653002600124653001200150653002600162653001600188653000900204653002400213653002100237653002500258100001400283700001300297700001900310245011100329300001000440490000900450520099700459022001401456       1984                            d  c1984 Jul-Aug10aAnimals10aConcanavalin A10aFemale10aImmunity, Cellular10aKiller Cells, Natural10aleprosy10aLymphocyte Activation10aMacrophages10aMice10aMice, Inbred BALB C10aMice, Inbred C3H10aMycobacterium leprae1 aRidel P R1 aJhol J S1 aKrahenbuhl J L00aCell-mediated immunity in mice treated with Mycobacterium leprae or with macrophages harbouring M. leprae.  a39-500 v135D3 a<p>Following treatment of BALB/c or C3H/HeN mice in the hind footpads with irradiated Mycobacterium leprae, a marked enhancement of natural killer (NK) activity was observed in cells from the draining popliteal lymph node or from the spleen. NK activity was further enhanced when the treatment consisted of killed M. leprae which had been incorporated into mouse peritoneal macrophages. This effect was noted as early as 2 weeks after treatment and persisted for at least 9 weeks. Lymphoblastic transformation in response to suboptimal doses of the T-cell mitogen transformation in response to suboptimal doses of the T-cell mitogen concanavalin A or to M. leprae antigen was assayed in parallel in cells from the draining popliteal lymph node and from the spleen. In contrast to NK assays, treatment with M. leprae alone moderately altered the response to mitogen. However, there was a prominent enhancement of the T-cell response when treatment consisted of M. leprae-laden macrophages.</p>  a0300-4910