01700nas a2200337   4500000000100000008004100001260001600042653002700058653002900085653002200114653001800136653001800154653002000172653004100192653002800233653001100261653001200272653001600284653002000300653001300320653000900333653001800342100001700360700001600377700001400393245012300407300001100530490000700541520080000548022001401348       1984                            d  c1984 Dec 3110aAntibodies, Monoclonal10aAntigen-Presenting Cells10aAntigens, Surface10aB-Lymphocytes10aCell Membrane10aHLA-DR Antigens10aHistocompatibility Antigens Class II10aHistological Techniques10aHumans10aleprosy10aLymphocytes10aPalatine Tonsil10aParaffin10aSkin10aT-Lymphocytes1 aCollings L A1 aPoulter L W1 aJanossy G00aThe demonstration of cell surface antigens on T cells, B cells and accessory cells in paraffin-embedded human tissues.  a227-390 v753 a<p>This paper describes a method for processing fresh tissue that allows immunohistological analysis on paraffin sections. The method is based on the use of periodate-lysine-paraformaldehyde fixation. The effects of variation in fixation time, concentration of paraformaldehyde, dehydration, clearing, wax embedding and enzyme treatment of cut sections were examined. An optimal processing procedure was established that retains good tissue morphology and allowed 21 out of 27 monoclonal antibodies tested to be used successfully on paraffin sections to identify all major cell subpopulations by their membrane antigenic characteristics. The value of this approach in studying the immunopathology of potentially dangerous infectious diseases and in leukaemia/lymphoma diagnosis is discussed.</p>  a0022-1759