01135nas a2200217   4500000000100000008004100001260001300042653001800055653001100073653001200084653002500096100001600121700001100137700001400148245008000162300001000242490000700252050003200259520061200291022001400903       1983                            d  c1983 Jul10aCulture Media10aHumans10aleprosy10aMycobacterium leprae1 aPrabhakar R1 aHari L1 aHerbert D00aObservations on the culture of M. leprae in Medium V. A preliminary report.  a450-40 v55  aInfolep Library - available3 a<p>This paper reports the preliminary findings of an investigation using Medium V for culture of M. leprae. The medium was prepared adhering to the procedures adopted by Veeraraghavan. Tissues from 7 cases of lepromatous leprosy (Bacteriological Index of 3 or more) were inoculated into Medium V and incubated at 8-10 degrees C for 72 hours. Quantitative estimation of leprosy bacilli were made employing the enumeration technique of Veeraraghavan. There was no evidence of multiplication in any of the cultures. The findings are in conformity with those of Kato (1983) and of Katoch and Desikan (1983).</p>  a0024-1024