02762nas a2200361   4500000000100000008004100001260001300042653001200055653001800067653001900085653002000104653001700124653001100141653002300152653001500175653001000190653001400200653001600214653000900230653002400239653002300263653002900286653003100315653001100346100001100357700001500368700001600383245014400399300001100543490000700554520182500561022001402386       1984                            d  c1984 Apr10aAnimals10aAscitic Fluid10aBlood Proteins10aCells, Cultured10aElectrolytes10aFemale10aHistocytochemistry10aHydrolases10aLiver10aLysosomes10aMacrophages10aMice10aMice, Inbred BALB C10aMice, Inbred C57BL10aMycobacterium Infections10aMycobacterium lepraemurium10aSpleen1 aHa D K1 aLawton J W1 aGardner I D00aExperimental murine leprosy: a biochemical study emphasizing lysosomal enzyme changes in vivo and enzyme secretion by macrophages in vitro.  a177-940 v403 a<p>This study was undertaken to identify biochemical alterations in serum, lymphoid organs, and peritoneal macrophages (PM) which reflect the histopathology of experimental Mycobacterium lepraemurium (MLM) infection in mice. A significant increase of acid phosphatase, beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, and lysozyme was found in serum, spleen, and liver homogenates of mice infected intraperitoneally (ip) with MLM. PM from infected mice showed a substantially greater rate of secretion of beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, and acid phosphatase than PM from normal mice. There was, however, no significant difference in the ability of PM from BALB/c and C57BL/6 mice to secrete such enzymes in vitro. There was also a significant increase in all these enzymes in PM in the early stage of infection but they dropped to values lower than normal in the advanced stage of infection despite the fact that such cells increased in size and protein content as the infection progressed. Infected mice were also found to have progressively elevated levels of serum lactic dehydrogenase, glutamic oxaloacetic, and glutamic pyruvic transaminases which indicated damages of hepatocytes and other tissues. Values of other blood components were also reported. Both BALB/c and C57BL/6 strain of mice, which are susceptible to the ip route of MLM infection, showed an indistinguishable pattern of biochemical alterations as reflected by their similar histopathological changes in various organs. BALB/c mice, which are still susceptible to subcutaneous (sc) route of infection showed similar characteristic changes in various serum components as before. In contrast, C57BL/6 mice, which are resistant to MLM infection sc, showed insignificant alterations in most of these biochemical parameters.</p>  a0014-4800