01838nas a2200289   4500000000100000008004100001260001300042653002700055653003500082653001400117653004100131653001100172653001200183653002000195653001500215653000900230653001800239100001800257700001600275700001500291700001100306245009300317300001000410490000700420520110700427022001401534       1983                            d  c1983 Mar10aAntibodies, Monoclonal10aFluorescent Antibody Technique10aGranuloma10aHistocompatibility Antigens Class II10aHumans10aleprosy10aLeukocyte Count10aPhagocytes10aSkin10aT-Lymphocytes1 aNarayanan R B1 aBhutani L K1 aSharma A K1 aNath I00aT cell subsets in leprosy lesions: in situ characterization using monoclonal antibodies.  a421-90 v513 a<p>Cryostat sections of dermal lesions from 30 untreated leprosy patients were studied by indirect immunofluorescence using monoclonal antibodies defining T cell subsets and Ia like antigens. Most lymphocytes in leprosy lesions were positive for OKT3 and Ia like antigens indicating thereby the presence of activated T cells. Maximal numbers of these cells were seen in localized paucibacillary tuberculoid leprosy lesions in close association with epithelioid cells. A decline in their numbers was observed over the leprosy spectrum with a marked reduction in disseminated, multi-bacillary, lepromatous leprosy where only scattered OKT3+ cells were visualized. OKT4 and OKT8 positive cells defining T cell subsets, were frequently found within the OKT3+ lymphocytes throughout the leprosy spectrum. The ratio of OKT4+/OKT8+ cells ranged from 1.2 to 5.0 in tuberculoid and from 0.2 to 1.0 in lepromatous lesions. Macrophages in the granulomas stained intensely with anti-Ia antisera. Ia like antigens were expressed to the same degree on macrophages with or without intracellular acid fast bacilli.</p>  a0009-9104