02506nas a2200349   4500000000100000008004100001260001300042653002700055653002200082653001400104653002300118653001100141653002800152653001800180653001200198653002700210653002900237653001800266653005700284100001500341700001500356700001600371700001600387700001300403700001500416700001200431245012600443300001200569490000800581520155300589022001402142       1984                            d  c1984 Jun10aAntibodies, Monoclonal10aAntigens, Surface10aGranuloma10aHistocytochemistry10aHumans10aImmunoenzyme Techniques10aInterleukin-210aleprosy10aReceptors, Immunologic10aReceptors, Interleukin-210aT-Lymphocytes10aTumor Necrosis Factor Receptor Superfamily, Member 71 aModlin R L1 aHofman F M1 aHorwitz D A1 aHusmann L A1 aGillis S1 aTaylor C R1 aRea T H00aIn situ identification of cells in human leprosy granulomas with monoclonal antibodies to interleukin 2 and its receptor.  a3085-900 v1323 a<p>Leprosy is a chronic granulomatous disease with an immunologic spectrum in which lepromatous leprosy patients have defective cell-mediated immune responses, in comparison to tuberculoid leprosy patients. Immunoregulatory aspects of this spectrum were investigated by using monoclonal antibodies to interleukin 2 (IL 2), IL 2 receptors (Tac), and T lymphocyte subpopulations with immunoperoxidase techniques on frozen sections of skin biopsy specimens from 10 tuberculoid and 10 lepromatous patients. A comparison of IL 2+ cells revealed markedly fewer IL 2+ cells in lepromatous specimens (lep. 0.028% +/- 0.02 vs tub. 0.46% +/- 0.28, p less than 0.001). These IL 2+ cells were large, exhibited cytoplasmic staining, and on double immunostaining were Leu-4+, Leu-3a+, Leu-2a-, Tac-, and OKT6-, consistent with the fact they are IL 2 producers. Equivalent numbers of Tac+ cells were observed in both lepromatous and tuberculoid granulomas (lep. 1.5% +/- 0.5 vs tub. 2.1% +/- 0.7, p, NS), suggesting that the responder cells are present in both conditions. The tuberculoid granuloma was highly organized, composed of a central core of mature macrophages, Leu-3a+ and Tac+ cells with a surrounding mantle of Leu-2a+, Leu-3a+, IL 2+, Tac+, and OKT6+ cells. In lepromatous granulomas, Leu-2a+, Leu-3a+, Tac+, and rare IL 2+ cells were randomly admixed with bacilli-laden macrophages. The defective cell-mediated immune responses in lepromatous leprosy appears to be associated with diminished IL 2 production and disorganization of the granuloma.</p>  a0022-1767