01909nas a2200337   4500000000100000008004100001260001300042653001800055653001400073653002000087653000800107653001100115653001800126653001300144653001200157653002600169653001600195653001400211653003600225653001800261100001100279700001600290700001400306700001600320700001500336245010300351300001000454490000700464520108600471022001401557       1984                            d  c1984 Dec10aCell Adhesion10aCell Line10aCells, Cultured10aDNA10aHumans10aInterleukin-210aKinetics10aleprosy10aLymphocyte Activation10aLymphokines10aMonocytes10aSuppressor Factors, Immunologic10aT-Lymphocytes1 aNath I1 aJayaraman J1 aSathish M1 aBhutani L K1 aSharma A K00aInhibition of interleukin-2 production by adherent cell factors from lepromatous leprosy patients.  a531-80 v583 a<p>Twenty-four hour supernatants (MoF) were obtained from monocyte rich 2 h adherent cells of 19 leprosy patients and four healthy contacts. MoF from borderline and lepromatous patients produced 52-61% inhibition of human interleukin-2 (IL-2) production by a PHA conditioned T cell line (Jurkat). Non-adherent cell supernatants and MoF from tuberculoid and healthy individuals had little effect on IL-2 production. The suppression effected by MoF was in the first 12 h of initiation of PHA stimulated Jurkat cell cultures. Suppressive MoF did not interfere with (1) IL-2 release, (2) IL-2 utilization by Con A-induced T cell blasts or (3) constitutive proliferation of Jurkat cells. Such MoF were released spontaneously from adherent cells of bacilliferous leprosy patients but required in vitro antigen triggering in long term treated lepromatous patients. It is possible that the unresponsiveness associated with lepromatous leprosy is related to the inhibition of IL-2 production by suppressive factors, thereby, preventing the further expansion of antigen reactive T cells.</p>  a0009-9104