02149nas a2200349   4500000000100000008004100001260001300042653001800055653001200073653001100085653002200096653002400118653001200142653002600154653001600180653001600196653001500212653001700227653001600244100001500260700001500275700001700290700001300307700001500320700001200335700001700347245014400364300001200508490000700520520125800527022001401785       1981                            d  c1981 Apr10aCell Movement10aDapsone10aHumans10aHydrogen Peroxide10aIn Vitro Techniques10aleprosy10aLymphocyte Activation10aLymphocytes10aNeutrophils10aPeroxidase10aPhagocytosis10aSuperoxides1 aAnderson R1 aGatner E M1 aRensburg C E1 aGrabow G1 aImkamp F M1 aKok S K1 aRensburg A J00aIn vitro and in vivo effects of dapsone on neutrophil and lymphocyte functions in normal individuals and patients with lepromatous leprosy.  a495-5030 v193 a<p>The effects of dapsone on polymorphonuclear leukocyte functions and lymphocyte mitogen-induced transformation were assessed in vitro and in vivo in normal individuals and in newly diagnosed untreated patients with lepromatous leprosy. The effects of dapsone on the cell-free generation of superoxide by the xanthine: xanthine oxidase system and iodination of bovine serum albumin by horseradish peroxidase were also investigated. In normal individuals dapsone mediated stimulation of polymorphonuclear leukocyte migration in vitro and vivo. Dapsone had no effect on postphagocytic hexose monophosphate shunt activity in vivo. Similar effects were found in patients with lepromatous leprosy. Dapsone also decreased the inhibitory activity of serum from patients with lepromatous leprosy on normal polymorphonuclear leukocyte migration in vitro. Progressive loss of serum-mediated inhibition of migration was observed after ingestion of dapsone by the patients. Further experiments showed that stimulation of polymorphonuclear leukocyte motility was related to inhibition of lymphocyte transformation at high concentrations in vitro, but had slight stimulatory activity on phytohemagglutinin-induced transformation in controls and patients in vivo.</p>  a0066-4804