00988nas a2200205   4500000000100000008004100001260001700042653001100059653001200070653002500082653001300107653002000120653002600140100001300166245008000179300001000259490000700269520049200276022001400768       1976                            d  c1976 Jul-Sep10aHumans10aleprosy10aMycobacterium leprae10aParaffin10aRosaniline Dyes10aStaining and Labeling1 aHarada K00aThe use of non-deparaffinized tissue sections for staining leprosy bacilli.  a366-80 v443 a<p>Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-carbol pararosanilin stain or methenamine silver stain for demonstrating mycobacteria.</p>  a0148-916X