01562nas a2200277   4500000000100000008004100001260001300042653002300055653002400078653001300102653001100115653001200126653002400138653002500162653003100187653002100218100001300239700001200252700001500264700001300279245010400292300001100396490000700407520085600414022001401270       1981                            d  c1981 Jun10aAntibody Formation10aAntigens, Bacterial10aEpitopes10aHumans10aleprosy10aMycobacterium bovis10aMycobacterium leprae10aPolysaccharides, Bacterial10aRadioimmunoassay1 aHarboe M1 aCloss O1 aReitan L J1 aDraper P00aDemonstration of antibodies reacting with different determinants on Mycobacterium leprae antigen 7.  a147-580 v493 a<p>A purified preparation of M. leprae antigen 7 was used to investigate the specificity of anti-M. Leprae 7 antibodies in leprosy sera in a radioimmunoassay. A solution containing arabinogalactan (AG) and arabinomannan (AM) inhibited the antibodies in some sera to a great extent, whereas the antibody activity was virtually unchanged in other sera under the same conditions. These findings indicate that the antibodies are directed against different determinants on M. leprae antigen 7. Antibodies against determinants other than AG and AM occurred particularly in lepromatous leprosy sera. In 12 out of 14 sera, AG and AM had similar inhibiting capacity. In one serum, AG had markedly higher inhibiting capacity than AM; in the last serum, the reverse was the case, demonstrating variation in anti-polysaccharide specificity in individual sera.</p>  a0148-916X