01535nas a2200337   4500000000100000008004100001260001300042653001000055653002400065653001800089653004400107653001300151653001100164653001100175653001900186653002100205653001700226653001200243653002600255653000900281653001600290653001800306653001800324100001400342700001300356245017800369300001100547490000700558520061800565022001401183       1982                            d  c1982 Dec10aAdult10aAntigens, Bacterial10aB-Lymphocytes10aDose-Response Relationship, Immunologic10aEpitopes10aFemale10aHumans10aHydrocortisone10aImmunoglobulin M10aIndomethacin10aleprosy10aLymphocyte Activation10aMale10aMiddle Aged10aMycobacterium10aT-Lymphocytes1 aFleming B1 aRook G A00aT-cell-dependent polyclonal activation by soluble mycobacterial extracts of B cells in peripheral blood mononuclear cell populations from leprosy patients and normal donors.  a589-950 v473 a<p>Soluble extracts of ultrasonically disrupted mycobacteria (including Mycobacterium leprae) cause strong T-cell-dependent, Cyclosporin A-sensitive, polyclonal B-cell activation, in human peripheral blood mononuclear cell preparations. It is not enhanced by cortisol. Cells from cases of lepromatous leprosy show no polyclonal B-cell activation in the presence of extracts of M. leprae, although they respond normally to other mycobacterial species. The polyclonal B-cell activation response to the M. leprae extract, of a small group of normal leprosy contacts, was reduced relative to that of non-contacts.</p>  a0019-2805